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Leica/3i Spinning Disk Confocal
Modular & customizable optics/microinjection

Model Information

  • Yokogawa Spinning Disk Confocal
  • Leica DMI6000 fully motorized inverted microscope (Marzhauser xy stage)
  • Metal-halide illuminator with fiber-optic coupling (long-life 2000 hr/bulb)
  • Extra illuminator and camera ports; fully modular and customizable (ask us!)
  • Semi-automatic nuclear and cytoplasmic microinjection of adherent cells with Eppendorf FemtoJet with integrated pump and Eppendorf Injectman 4

 Leica SDC

Specifications

  • Yokogawa CSU10 spinning Nipkow disk with microlenses
  • Leica DMI6000 fully motorized platforms
  • Solid-state laser lines at 405, 488, 568 and 647 nm (>50mW each line into SDC)
  • Metamorph software for intuitive data acquisition

Applications

  • Optical sections or image stack with high contrast and definition
  • Best for moderate and bright fluorescence
  • Low rate photobleaching/ extended cell viability
  • Minute details of points and edges
  • Simple-to-use, easy software
  • Quantitative 3D analysis
  • Quantitative colocalization analysis

Examples


Protein in nuclei of Drosophila embryo


Pole cells in Drosophila germarium


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