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Nikon Eclipse Ti-E
for Total Internal Reflection Fluorescence (TIRF) Microscopy

Applications

  • Widefield or TIRF fluorescence imaging of membrane proteins, receptor proteins, signalling molecules, cortical cytoskeleton proteins

Special Capabilites

  • TIRF illumination exciting molecules within 100 nm of the coverslip surface
  • integrated Perfect Focus System to address focus drift with time-lapse imaging
  • Photometrics Evolve EMCCD camera: Absolute sensitivity calibration (photon-counting), single-molecule detection with Quant-view and Background Event Reduction Technology (BERT)
  • Simultaneous acquisition of two spatially identical but spectrally distinct images using Photometrics Dual-View

System Information

Microscope Model: Nikon Eclipse Ti-E
Camera Model: Photometrics Evolve 512 EMCCD
Imaging Software: Nikon NIS-Elements v3.22.00
Accessories available: Tokai-Hit environmental chamber (CO2, humidity, 37C temperature)

Microscope Specifications

Objectives
Installed 20x/0.45 S Plan Fluor (dry)
40x/0.60Ph2 S Plan Fluor (dry)
100x/1.49 Apo TIRF (oil)
Available by request 40x/1.3 Plan Fluor (oil)
60x/1.4 Plan-Apo (oil)
100x/1.4 Plan-Apo (oil)
 
Filter sets
Eyepiece View CFP
YFP
Camera View
(both TIRF & Widefield, filter-wheel on emission)
CFP, YFP, CFP-YFP FRET
GFP, RFP, GFP-RFP FRET
Dual-View CFP/YFP
GFP/RFP
   
Laser Lines
CFP 445nm (40mW Diode laser)
not standard cube 457nm (Argon laser)
not standard cube 477nm (Argon laser)
GFP 488nm (Argon laser)
YFP 514nm (Argon laser)
RFP/dSTORM 561nm (0.5W fiber laser)
AF647/dSTORM 647nm (0.5W fiber laser)

 


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