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Microscope Facility Events & Workshops

  • Feb 22 - Mar 3, 2016 Biophysics B3
    Zeiss Airyscan Super-Resolution Demo It's an easy-to-use super-resolution technique (~170nm resolution xy) that improves every confocal image, regardless of objective or depth. The side-by-side compareisons are stunning!
    Seminar: Xufeng Wu Traylor 709 (Talbot Library), School of Medicine, simulcast to Clark Hall 100, Homewood
    Xufeng Wu (NIH HLBI), director of a microscopy core at the NIH, has almost all the super-resolution techniques at her facility and teaches a course on super-resolution. She will give a user's perspective of the Airyscan.
    Recording of seminar
  • Oct 12, 2015-Nov 20, 2015 Rescheduled for later
    3i Lattice Light-Sheet Microscope (LLSM) Installation & Training
       Invented by Nobel-laureate Eric Betzig, the LLSM is ideal for high-resolution 3D imaging of fluorescence samples with almost no phototoxicity. Partnered with 3i, we will have one of the first beta-instruments and will help develop protocols and customizations for biomedical research applications.
    Oct 12, 2015 Rescheduled for later Installation of microscope
    Oct 19, 2015-Nov 20, 2015 Rescheduled for later Early Users: (Grant Users; others must get prior approval)
       LLSM training/testing schedule (no cost to Grant Users for group training)
  • Dec 11, 2013-Feb 7, 2014
    Zeiss microscope upgrades & replacements
    Dec 11, 2013 installation done: LSM700
       LSM700 re-training schedule (no cost to Meta users)
    Jan 21, 2014 Meta1 removed: renovate room for 780
    Jan 27, 2014 installation done: 3iSDC
       Definite Focus: for training
    Feb 7, 2014 installation done: LSM780FCS
       LSM780 training & testing schedule (no cost to Grant Users for group training)
  • Feb 11-27, 2013 PCTB, room 207
    Zeiss Elyra S.1 Super-Resolution Demo It's an easy-to-use super-resolution technique (~120nm resolution xy) that requires minimal sample constraints, while outperforming normal confocal microscopy. It is the ONLY super-resolution technique that can operate with deep specimens (up to ~30um) AND IMPROVES RESOLUTION in ALL THREE DIMENSIONS. Guideline for sample preparation and event overview are available.
  • Nov 7-9, 2011 (CHANGE in date) PCTB, room 207
    Leica GSD Super-Resolution Demo Just-released 'Ground-State Depletion' super-resolution instrumentation. Almost protein-sized (~10nm) resolution for individual fluorophores. Great for fixed/permeabilized samples or extracellular markers. Conditions for intracellular still being developed. Documentation for overview and for protocols, as well as some reagents, are available for participants.
  • Nov 7-8, 2011 (CHANGE in location) ~600 Washington St (by McElderry St, bus)
    GE High-Content Screening and API Deconvolution Microscope In the GE Healthcare bus, two instruments will be visiting JHU SOM. One is a high-content/high-throughput imaging system to screen multiple microscopic samples by automated image recognition software. The other is an automated deconvolution microscope great for monitoring living cells while minimizing phototoxicity, often better than confocal in quality. It's the next generation offering from Applied Precision.
  • Mon Oct 17, 2011, 2:00-3:00 Bodian Conf Room, 1830 E Monument St, Suite 2-200
    Seminar: Super-Resolution by Ground-state Depletion (GSD) Similar resolution as PALM/STORM, but NOT PALM/STORM and NOT STED. Some constraints on specimen preparation, but necessary introduction for the Demo next month (on Nov 7-Nov 9, 2011).
  • June 23, 2011, 1:30-4:30 WBSB West Lecture Hall
    Nuance, inForm & Trio Demo James R. Mansfield (Caliper Life Sciences) will present multi-spectral imaging without confocal (great for histology, tissues & quantitation of protein expression)
  • May 25, 2011, 2:00-3:00 Bodian Conf Room, 1830 E Monument St, Suite 2-200
    Seminar on using high-pressure freezing and freeze-substitution Kim Rensing (Leica Microsystem) will introduce the Microscope Facility's newest acquisitions: HPM100 (high-pressure freezer) and AFS2 (automated freeze-substitution).  Becoming the gold-standard of specimen preparation, these techniques preserve cellular ultrastructure with great fidelity.
  • May 9-May 19, 2011 PCTB Rm 207
    Leica CW-STED Super-resolution Demo (2nd visit) Just-released detector improvement: Outstanding signal-to-noise detectors {"HyD" hybrid detectors, ~5x better S/N}; More usable dyes.
  • July 8, 2010 - July 21, 2010 Montcastle Aud & PCTB G18/19
    Leica CW-STED Demo Try the newest version of STED super-resolution microscopy: new fluorescent protein compatibility and new 2-color colocalization at super-resolution
  • Sept 10, 2009, 10-12 noon outside Microscope Facility (Physiology G04)
    Invitrogen Troubleshooting Office Hours Premo FUCCI (fluorescenlty monitor cell-cycle in living cells) and new quantum dot (QDot) compatible products
  • May 20, 2009; 11:30-1:30 outside Microscope Facility (Physiology G04)
    Invitrogen Troubleshooting Office Hours Click-It TUNEL (apoptosis indicator) and APEX labeling (small amts of protein)
  • Apr 23, 2009; 1-3PM outside Microscope Facility (Physiology G04)
    Invitrogen Troubleshooting Office Hours EdU (better than BrdU), Alexa Fluors, Prolong Gold Antifade

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