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Fees to use the Microscope Facility
effective July 1, 2018 - June 30, 2019 (FY19)

For regulatory reasons, we must change our fee structure (see full description of changes). Effective July 1, 2018, 12-month commitments (membership subscriptions) are no longer required for self-use of equipment. In addition, a new access fee to provide data storage and image processing/analysis software (previously included with membership subscriptions) will be charged monthly and only when image data is collected or processed; the amount of the fee is tiered and depends on usage that month. Costs for supplies will remain unchanged from last year. The FY19 rates effective July 1, 2018 are listed below (see prior rates).

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Staff-operated/staff-supervised Equipment Use JHU/Academic Fast-Forward Corporate Other Corporate
Staff-operated: All microscopes, all equipment, all software
Except: Lattice Light-Sheet (LLSM)
$130/hr $156/hr $260/hr
Staff-supervised, user-operated ("semi-assisted": untrained users or users during training practise) $65/hr $78/hr $130/hr
Lattice Light-Sheet Microscope (LLSM): only available as staff-operated
special subsidy from Dean of Research for JHU users
$29/hr $156/hr $260/hr
Unassisted Equipment Use (Self-Use)
Electron microscopes
Includes: Hitachi H7600 TEM, LEO FE-SEM, Philips CM120 BioTwin TEM
$29/hr $34.80/hr $58/hr
Confocal & specialized light microscopes
Only: 3i/Live-cell SDC, GE OMX Super-Resolution, Nikon TIRF/dSTORM Super-Res, Zeiss LSM510Meta, Zeiss LSM700, Zeiss LSM710NLO multiphoton, Zeiss LSM780FCS, Zeiss LSM800GaAsP
Except: High-Content Imager (IXM), Lattice Light-Sheet (LLSM), Leica SDC/Microinjector (SD4)
$29/hr $34.80/hr $58/hr
Long-term time-lapse confocal microscopy
(3h minimum, overnight 9p-9a & weekend/holidays)
$10/hr $12/hr $20/hr
After-hoursNights 7p-9a & weekends
rate for
highly-experienced confocal users>500h experience on Facility confocals

Rate only for confocal use at night (7p-9a) and on weekends. To qualify, users must log >500h experience on Facility confocals, excluding periods of long-term time lapse (see above).
$10/hr $12/hr $20/hr
MolDev High-Content Imager
Leica Spinning Disk Confocal/Microinjection
(promotional rates)
$16/hr $19.20/hr $32/hr
Basic light microscopes
Only: Olympus Color Histology (OLC), Olympus Inverted/Ratiometric (OL1), Olympus Upright (OL2), Nikon Fluor Dissecting (NKD)
$16/hr $19.20/hr $32/hr
Specimen preparatory equipment: Cryostat, Ultramicrotome
Supplies are not included; bring your own or purchase from us.
$16/hr $19.20/hr $32/hr
Training: all equipment, all image analysis software
(maximum 2 trainees per session; typical examples in gray italics)
Base rate:
Basic Confocal (3h):
Adv/Add'l Confocal (2h):
Software (1h):
$130
$390
$260
$130
/hr
/sess
/sess
/sess
$156
$468
$312
$156
/hr
/sess
/sess
/sess
$260
$780
$520
$260
/hr
/sess
/sess
/sess
Certification (test to allow unassisted self-use) $29/15 min $34.80/15 min $58/15 min
Data access & usage fee (monthly; tiers for monthly usage of
image-generating equipmentAll microscopes; excludes specimen prep, such as cryostat
)
(Fee provides access to temporary storage of image data, unlimited access to computer workstations, unlimited access to image analysis software)
Tiers
≤5h/mo:
≤10h/mo:
>10h/mo:

$45
$65
$85

/mo
/mo
/mo

$54
$78
$102

/mo
/mo
/mo

$90
$130
$170

/mo
/mo
/mo
Custom Projects: Specimen Preparation, Imaging & Software Services
Custom specimen preparation and imaging services (consultation meeting required)
Lab supplies included, except for high-pressure freezer, for primary antibodies, and for uranyl formate
$130/hr $156/hr $260/hr
Custom software and analysis development services (consultation meeting required)
MatLab and Fiji/ImageJ scripts; ask about other software packages.
$130/hr $156/hr $260/hr
Supplies: uranyl formate (special TEM staining)
Frozen in single-use aliquots: an "experiment" is sufficient for 10 grids.
$25/expt $30/expt $50/expt
Supplies: high-pressure freezer (HPF)
See below for explanation of Session Fees1. Specimen supplies are sufficient for standard quadruplicate freezing of each specimen. Fees do not include labor for specimen preparation and their freezing or freeze-substitution (labor extra).
 $200
$30
/session1
/specimen
 $240
$36
/session1
/specimen
 $400
$60
/session1
/specimen
Supplies: automated freeze-substitution (AFS)
See below for explanation of Session Fees1. Fees do not include labor for subsequent processing or additional liquid nitrogen.
 $115
/session1  $138
/session1  $230
/session1
 1Session fees of HPF and AFS includes all liquid nitrogen (LN2) and labor to prepare equipment with full LN2 capacity before session. Lots of time and LN2 is required. Costs of subsequent labor and additional liquid nitrogen are extra. Session fees can be shared between users of the same session. Typically Thursdays, contact us for the schedule of HPF sessions.

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