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Fees to use the Microscope Facility
effective Jul 1, 2021 - June 30, 2022 (FY22)

Rates for most self-usage will drop >3%, and all other rates will be the same as last year. The FY22 rates effective July 1, 2021 are listed below (see prior rates).

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Staff-operated/staff-supervised Equipment Use JHU/Academic Fast-Forward Corporate Other Corporate
Staff-operated: All microscopes, all equipment, all software
Except: Lattice Light-Sheet (LLSM)
$130/hr $156/hr $260/hr
Staff-supervised, user-operated ("semi-assisted": untrained users as part of TEM projects) $65/hr $78/hr $130/hr
Lattice Light-Sheet Microscope (LLSM): only available as staff-operated
Promotional rate for JHU users only
$28/hr $156/hr $260/hr
Unassisted Equipment Use (Self-Use)
Electron microscopes
Includes: Hitachi H7600 TEM, LEO FE-SEM
Except: ThermoFisher Talos L120C Cryo-TEM
$28/hr $34/hr $56/hr
ThermoFisher Talos L120C Cryo-TEM $45/hr $54/hr $90/hr
Confocal & specialized light microscopes
Only: 3i/Live-cell SDC, GE OMX Super-Resolution, Nikon TIRF/dSTORM Super-Res, Zeiss LSM700, Zeiss LSM710NLO multiphoton, Zeiss LSM780FCS/Quasar, Zeiss LSM800GaAsP, Zeiss LSM880 Airyscan Fast
Except: High-Content Imager (IXM), Lattice Light-Sheet (LLSM), Lumicks C-Trap, Leica SDC/Microinjector (SD4), Zeiss LSM880 Airyscan Fast (880)
$28/hr $34/hr $56/hr
Long-term time-lapse confocal microscopy
(3h minimum, overnight 9p-9a & weekend/holidays)
$9
$14
/hr
/hr (LSM880)
$11
$16
/hr1
/hr1 (LSM880)
$18
$28
/hr1
/hr1 (LSM880)
After-hoursNights 7p-9a & weekends
rate for
highly-experienced confocal users>500h experience on Facility confocals

Rate only for confocal use at night (7p-9a) and on weekends. To qualify, users must log >500h experience on Facility confocals, excluding periods of long-term time lapse (see above).
$9
$14
/hr
/hr (LSM880)
$11
$16
/hr1
/hr1 (LSM880)
$18
$28
/hr1
/hr1 (LSM880)
Zeiss LSM880 Airyscan Fast
Temporary (Jan 1, 2021-Feb 28, 2022): Recover costs for repairing excessive damage (4 of 6 objectives broken in 2020)
$36/hr $44/hr $72/hr
MolDev High-Content Imager
Leica Spinning Disk Confocal/Microinjection
(promotional rate for JHU users only)
$15/hr $34/hr $56/hr
Lumicks C-Trap (optical trap/confocal)
(promotional rate for JHU users only)
$10/hr $34/hr $56/hr
Basic light microscopes
Only: Olympus Color Histology (OLC), Olympus Inverted/Ratiometric (OL1), Olympus Upright (OL2), Nikon Fluor Dissecting (NKD)
$15/hr $18/hr $30/hr
Specimen preparatory equipment: Cryostat, Ultramicrotome
Supplies are not included; bring your own or purchase from us.
$15/hr $18/hr $30/hr
Training: all equipment, all image analysis software
(typical examples in gray italics)
Base rate:
Basic Confocal (3h):
Adv/Add'l Confocal (2h):
Software (1h):
$130
$390
$260
$130
/hr
/sess
/sess
/sess
$156
$468
$312
$156
/hr
/sess
/sess
/sess
$260
$780
$520
$260
/hr
/sess
/sess
/sess
Certification (test to allow unassisted self-use) $28/15 min $34/15 min $56/15 min
Data resources fee (monthly; tiers for monthly usage of
image-generating equipmentAll microscopes; excludes specimen prep, such as cryostat
)
(Fee provides for temporary network storage of image data, and unlimited access to computer workstations and image analysis software during the month)
Tiers
≤5h/mo:
≤10h/mo:
>10h/mo:

$30
$50
$70

/mo
/mo
/mo

$36
$60
$84

/mo
/mo
/mo

$60
$100
$140

/mo
/mo
/mo
Software & workstation fees (for months without usage of
image-generating equipmentAll microscopes; excludes specimen prep, such as cryostat
)
$5/hr $6/hr $10/hr
 1After-hours access to the campus buildings must be provided by other JHMI entities. The Microscope Facility cannot assume the responsibilities implicit in providing campus access to non-JHU users.
Custom Projects: Specimen Preparation, Imaging & Software Services
Custom specimen preparation and imaging services (consultation meeting required)
Lab supplies included, except for high-pressure freezer, for primary antibodies, and for uranyl formate
$130/hr $156/hr $260/hr
Custom software and analysis development services (consultation meeting required)
MatLab and Fiji/ImageJ scripts; ask about other software packages.
$130/hr $156/hr $260/hr
Supplies: uranyl formate (special TEM staining)
Frozen in single-use aliquots: an "experiment" is sufficient for 5 grids.
$25/expt $30/expt $50/expt
Supplies: high-pressure freezer (HPF)
See below for explanation of Session Fees1. Consumables for each freeze do not include sapphire coverslips (only aluminum planchettes). Fees do not include labor for specimen preparation and their freezing or freeze-substitution (labor extra).
 $200
$10
/session2
/freeze
 $240
$12
/session2
/freeze
 $400
$20
/session2
/freeze
Supplies: automated freeze-substitution (AFS)
See below for explanation of Session Fees1. Fees do not include labor for subsequent processing or additional liquid nitrogen.
 $115
/session2  $138
/session2  $230
/session2
 2Session fees of HPF and AFS includes all liquid nitrogen (LN2) and labor to prepare equipment with full LN2 capacity before session. Lots of time and LN2 is required. Costs of subsequent labor and additional liquid nitrogen are extra. Session fees can be shared between users of the same session. Typically Thursdays, contact us for the schedule of HPF sessions.

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