Zeiss LSM700
Single-point, laser scanning confocal microscope
Model Information
- Zeiss AxioObserver with LSM700 confocal module
- Cite in your manuscripts (example text): NIH Grant S10OD016374
(Institute:Office of the Director [OD], Office of Research Infrastructure Programs [ORIP])
Installed: Dec 11, 2013
Specifications:
- Zeiss AxioObserver inverted microscope with LSM700 confocal module
- Portable 37°C heater for 35mm dishes or chamber slides also available
- NA 0.55 condenser (dry only) with DIC for 20X, 40X, 63X and 100X lenses
- Objectives:
- 10X/0.30 PlanNeofluar (dry, no DIC)
- 20X/0.80 Planapo (dry, no DIC)
- 40X/1.30 PlanNeofluar oil, with DIC
- 40X/1.20 C-Apo water, with DIC, upon request
- 63X/1.4 PlanApo oil, with DIC
- 100X PlanApo oil, with DIC, upon request
- 4 laser lines available:
- 405 (diode laser),
- 488 (solid-state)
- 561 (solid-state)
- 639 (diode laser)
- X-Cite 100W metal-halide illuminator for wide-field fluorescence
- Fluorescence filter sets (wide-field; non-confocal):
- #49 for DAPI, #38 for GFP, #43 For Cy3
- Latest Zen software to control microscope and confocal
- Zen software on a separate workstation for image analysis.
- Download Zen LE software available for desktop computers in your lab
- Analysis with Volocity, Imaris or ImageJ on our analysis workstations
- Use LOCI BioFormats to import images into ImageJ
Applications
- Single confocal images or image stacks for 3D viewing
- FRAP with user-defined photobleach marks
- Photoactivation at 405 nm of caged dyes and other molecules
- Colocalization of different fluorescent signals
- Speed and versatility
