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Microscope Facility News & Equipment Status

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2022-08-08: Server Upgrade: TempStorage4 Still Offline, Security Issue

You may have encountered issues with accessing TempStorage4. Unfortunately, the server transition is done but there is a security issue that we are trying to resolve. BSNO is investigating. Thank you for your patience.

08/04/2022 03:40 PM >> Subj: Server Upgrade: TempStorage4 Offline from 2-4pm on Friday, August 5
The Microscope Facility server will be unavailable for approximately 2 hours from 2pm to 4pm on Friday, Aug 5 to allow upgrading to larger capacity. All data will be transferred at time of upgrading; our intentions are to make the upgrade transparent, but it would be prudent to confirm the integrity of your data.

Specifically, users should avoid using the server during the downtime. In particular, they should never save live microscope images directly to the server, and should be saving to the local computer drives for subsequent transfer to the server. If they are saving live data directly to the server, software may freeze and data will get lost/corrupted when the server goes offline.

If users are unsure if they are doing this dangerous practice and their reservation spans the start of the downtime, they should contact the staff (microscopy@jhmi.edu)

Thank you for your patience.

2022-08-08 (ThermoFisher Talos L120C): ThermoFisher Talos: Back online and calendar opened again

The Talos is operational! The artifact on the upper left corner of the camera has been corrected. Please let us know if you have any questions or need additional information.
Thank you for your patience and understanding!
Barbara


07/02/2022 11:10 AM >> Subj: ThermoFisher Talos: Vacuum Down
The ThermoFisher service engineer will be on-site to replace a part to get the system back under vacuum. The artifact in the upper left corner of the Ceta camera has been corrected (service 6/29-7/1). An update will be sent as soon as the TEM is operational. Thank you for your patience.

2022-07-28 (Zeiss LSM 880 Airyscan FAST): Zeiss 880 back online; room temperature and humidity have stabilized

The 880 is operable now. The room temperature and humidity have stabilized. Thank you for your patience.

07/28/2022 10:21 AM >> Subj: Zeiss 880 offline; high temperature and humidity
The Zeiss LSM880 is down and the failure may be attributed to the environment - high temperature and humidity. We will monitor it and report again tomorrow morning. I will cancel unused reservations during the period (7/28-8/1).

2022-07-28 (Leica Cryostat): Leica Cryostat: Cable replaced and cryostat available for use

The annual maintenance and repairs have been completed on the cryostat. It is now open for reservations. Thank you for your patience.

07/25/2022 09:14 AM >> Subj: Leica Cryostat: Cable repair
The Leica Cryostat is not usable until we get a replacement cable for the microtome head. We are awaiting repair and will update users as soon as we have a replacement. Apologies for the inconvenience and thank you for your patience.

2022-07-08 (Zeiss LSM 780 FCS): Zeiss 780 back online; main controller working again

The 780 is operational again. Thank you for your patience.

07/07/2022 02:52 PM >> Subj: Zeiss 780 offline; awaiting service
The 780 system is not working right now. Both Hoku and LaToya are trying to troubleshoot and remedy the issues. A service request will be placed with Zeiss.

2022-06-21 (GE OMX SR (SIM)): GE OMX OFFLINE - stage stopped moving

The OMX stage has stopped moving. I've contacted service for repair, and will let you know when the repair is scheduled. Apologies for the inconvenience. -LaToya

2022-06-15 (Zeiss 710NLO (multiphoton)): 2P NDD deflector plate BROKEN on LSM710 --temporary replacement installed

LSM710 users: Unfortunately, our NDD deflector plate was broken today. This means that any 2-photon imaging which uses the NDDs cannot be done. We have contacted Zeiss and ordered a replacement. We will update you when it arrives. We have fashioned a replacement that uses an older dichroic, which will allow you to continue to use the system as normal, but you may need to use slightly higher laser power or detector gain to achieve the same brightness.

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