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Microscope Facility News & Equipment Status

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2019-10-17 (Leo FESEM): Leo FESEM is offline until further notice

The SEM is down until further notice. The facility staff are troubleshooting a vacuum leak.

2019-10-15 (Nikon TIRF (dSTORM)): TIRF: 560nm laser repaired, reinstalled & realigned

10/15/2019 1:12pm (UPDATE) >> The 560nm laser was repaired by the manufacturer and reinstalled on the Nikon TIRF system. Hoku has realigned the lasers and it the system is fully functional. As a precaution, please adjust your settings if needed.

Thank you all for your patience.

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9/04/2019 6:15pm >> *UPDATE* The laser has been sent to the manufacturer and we estimate will be returned in early October.

****************************************We are experiencing a startup issue with the 560nm laser (for red fluorescence) on the Nikon TIRF. We are troubleshooting and will provide updates when information becomes available. For now, please plan your experiments accordingly.

2019-09-30 (Zeiss LSM 880 Airyscan FAST): Zeiss 880: Special 63x lens removed for repair

The 63x on the Zeiss LSM 880 will be removed Tuesday, October 1 for repair. The channel alignments are not within specification due to suspected damage. While this objective is being evaluated, a 63X objective is being removed from another system to temporarily replace the special 63x. We apologize for the inconvenience but would also like to remind all users that these objectives are very expensive and time consuming to repair. Please be a good community user and exercise caution around all the microscopes. Barbara J. Smith EM Specialist JHU SOM Microscope Facility Physiology G04 Baltimore, MD 21205 410-614-6890 410-614-2086

2019-09-18 (Zeiss LSM 880 Airyscan FAST): Zeiss 880: 633 laser (laser head and power supply replaced)

9/18/2019 9:55am (UPDATE) >> The laser head for the 633 laser was replaced on Tuesday (9/17) by the Zeiss engineer. Thank you all for your patience.

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9/18/2019 9:53am >> CORRECTION: The power supply for the 633 laser on the 880 system was replaced in August.

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7/26/2019 9:46am >> The 633 laser on the 880 system has been replaced. The new module has slightly lower output, so you may have to turn up the power slightly to achieve the same brightness as before in far-red.

2019-09-13 (Workstation 4 (ThunderSTORM, Microvolution),Workstation 6 (Arivis/LLSM),Workstation 7 (AcuityXpress/MetaExpress)): Imaris inaccessible during Rangos outage

The Imaris software will not be accessible from the Microscope Facility workstations from 5pm Friday (Sept 13) to 9am Monday (Sept 16) due to the electrical outage scheduled in Rangos where the Imaris server is housed.

2019-09-13 (Workstation 1 (Imaris/Volocity/Zen),Workstation 2 (Elements/Imaris),Workstation 3 (Slidebook/Imaris/ZenFCS)): Imaris inaccessible during Rangos outage

The Imaris software will not be accessible from the Microscope Facility workstations from 5pm Friday (Sept 13) to 9am Monday (Sept 16) due to the electrical outage scheduled in Rangos where the Imaris server is housed.

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