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2017-01-10 (Zeiss LSM 700 Confocal): Zeiss LSM700 moving down the hall to Biophysics B3

The Microscope Facility Zeiss LSM700 will be relocated to Biophysics B3, just down the hall from its current location (Physiology G09) to make way for the new Zeiss LSM800GaAsP. We anticipate that the 700 system will be offline from 10am-2pm Thursday (1/12) and again on Friday morning (1/13).

All certified 700 users will have access to the new room which has 24/7 swipe access. Please remember to have your badge when you come to image on the 700.

More information about transitioning users to the new microscope system, LSM800GaAsP, will be posted soon. Thank you for your cooperation.

Microscope Facility News & Equipment Status

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2017-05-18 (3i Spinning Disk Confocal,Leica EM ICE High Pressure Freezer,Leica EM UC7 Cyro-ultramicrotome): May 19-20 >> LIMITED FACILITY ACCESS (Phys G05/3i Confocal)

UPDATE: The Housekeeping staff was unable to complete its task of stripping and polishing the hallway floor outside Physiology G04, G05 and G06. PLEASE DO NOT ENTER THIS AREA Friday evening (May 19) through Saturday morning (May 20). Thank you for your cooperation.

2017-05-10 >> Next week, on Tuesday evening (into Wednesday morning, May 16-17) and Wednesday evening (into Thursday morning, May 17-18), Housekeeping will be stripping the floors on the ground level of the Physiology Building. Please do not try to access the area during that time that the instrument is blocked off on the reservation calendar. Please allow the Housekeeping staff to complete the task. I will post a reminder on the equipment rooms and hallway. Thank you for your understanding.

2017-05-17: TempStorage3 Alternative Access

Because of the 'Wanna.Cry' ransomware attack, Enterprise services turned off some internet protocols earlier this week (probably 5/16/2017). As a result, Enterprise-managed computers and VPN access (on 5/20/2017) can no longer access TempStorage3 directly. Non-managed computers (e.g. Basic Sciences) are unaffected. You are on an Enterprise-managed computer system if you have to log onto your computer using JHED credentials.

As a work-around, we have enabled FTP access to TempStorage3. Use your web browser (Internet Explorer, Firefox, Chrome, etc) and use the URL ftp://162.129.230.229/TempStorage3/. For security, I can’t post the login credentials in plain text here. Instead, go to https://jh.box.com/s/pnuvp562ny7jeulxy6l034wk8t6r8ea2 (JHBox; enter your JHED credentials to open link) to get the user name and password.

Our long-term solution is to replace TempStorage3 with a newer server that has more secure internet protocols available. However, that may take Basic Science Network Office people much more than a month to purchase, receive and install because they have to deal with >40 servers in Basic Sciences departments.

2017-05-15 (Philips CM120 TEM): Philips TEM OFFLINE: ODP water/circuit message

This past weekend (May 13-14), the Philips TEM users reported that the instrument shut down and displayed the message "ODP water," and now we have an error message about the ODP circuit. Barbara checked the water and a few other things, but it has now escalated to an FEI service call.

2017-05-11 (Zeiss LSM 510-meta #2): Zeiss 510-meta OFFLINE: issue resolved, new operational procedure

5/11/2017 11:08am (UPDATE)>>
The safety issue has been resolved, but a new operational procedure will need to go in place before the 510 is back online for users. Thank you for your patience!

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5/4/2017>>The Zeiss 510-meta is not available for acquisition due to an unknown safety issue. We are trying to resolve it with Zeiss but have not yet been able to do so. Files saved on the local temp storage will not be deleted while the microscope is inoperable. We are sorry for the inconvenience.

2017-05-10 (Workstation 1 (Zen/Imaris/VolocityDecon),Workstation 2 (Elements/Imaris/Volocity),Workstation 3 (Slidebook/Imaris/ZenFCS)): May 16-18 >> LIMITED FACILITY ACCESS (Analysis Workstations)

Next week, on Tuesday evening (into Wednesday morning, May 16-17) and Wednesday evening (into Thursday morning, May 17-18), Housekeeping will be stripping the floors on the ground level of the Physiology Building. Please do not try to access the area during that time that the instrument is blocked off on the reservation calendar. Please allow the Housekeeping staff to complete the task. I will post a reminder on the equipment rooms and hallway. Thank you for your understanding.

2017-05-10 (Leica Cryostat,Nikon Fluorescence Dissection w SLR,Olympus Color Station): May 16-18 >> LIMITED FACILITY ACCESS (Color/ Dissection/Cryostat)

Next week, on Tuesday evening (into Wednesday morning, May 16-17) and Wednesday evening (into Thursday morning, May 17-18), Housekeeping will be stripping the floors on the ground level of the Physiology Building. Please do not try to access the area during that time that the instrument is blocked off on the reservation calendar. Please allow the Housekeeping staff to complete the task. I will post a reminder on the equipment rooms and hallway. Thank you for your understanding.

2017-05-10 (Hitachi 7600 TEM,Leo FESEM,Philips CM120 TEM): May 16-18 >> LIMITED FACILITY ACCESS (EM lab/equipment)

Next week, on Tuesday evening (into Wednesday morning, May 16-17) and Wednesday evening (into Thursday morning, May 17-18), Housekeeping will be stripping the floors on the ground level of the Physiology Building. Please do not try to access the area during that time that the instrument is blocked off on the reservation calendar. Please allow the Housekeeping staff to complete the task. I will post a reminder on the equipment rooms and hallway. Thank you for your understanding.

2017-05-10 (Zeiss LSM 510-meta #2,Zeiss LSM 780 FCS,Zeiss LSM 800 GaAsP): May 16-18 >> LIMITED FACILITY ACCESS (800/780/510)

Next week, on Tuesday evening (into Wednesday morning, May 16-17) and Wednesday evening (into Thursday morning, May 17-18), Housekeeping will be stripping the floors on the ground level of the Physiology Building. Please do not try to access the area during that time that the instrument is blocked off on the reservation calendar. Please allow the Housekeeping staff to complete the task. I will post a reminder on the equipment rooms and hallway. Thank you for your understanding.

2017-04-19 (Workstation 6 (AcuityXpress/Microvolution)): WK6, memory upgrade.

4 memory sticks installed by Hoku.

2017-04-05 (MolDev IXM High Content Imager,Workstation 6 (AcuityXpress/Microvolution),Zeiss LSM 700 Confocal): Biophysics B3, Equipment BACK ONLINE (700/IXM/3iLLS/WK6)

4/6/2017 10:27am (UPDATE)>>
The equipment (700/IXM/3iLLS/WK6) have been inspected and are back online. Thank you for your patience!

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4/6/2017 9:44am (UPDATE)>>
CORRECTION: Facilities has completed the work in B3 but the equipment (700/IXM/3iLLS/WK6) are still offline. Thank you for your patience!

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4/6/2017 9:25am (UPDATE)>>
The equipment in Biophysics B3 (700/IXM/3iLLS/WK6) are ready to use again. Thank you for your patience!

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The equipment in Biophysics B3 is offline today as of now. Water is leaking from the ceiling in adjacent rooms with some minor water leaking in B3. Facilities is attending to the issue now but our access to the room is restricted. Once we are given clearance, we will inspect all the instruments and bring them back online. I will try to update you as often as possible, and when the situation has changed.

We apologize for the inconvenience. If you are a 700 user want access the 510 but cannot reserve it, please email microscopy@jhmi.edu for 510 access.

Loza

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