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2017-07-14: Problems getting to TempStorage4?

Although most of our users have transitioned to TempStorage4 successfully (see FastFacts/Network for instructions), some are still having issues.

Troubleshooting:

Try to use a different computer to access TempStorage4. Factors that might matter are:

  1. Network type: WiFi (NOT GuestNet!) vs Wired Ethernet (preferred)
  2. Operating system: Windows vs Mac, which version (NOT WinXP!)?
  3. Computer: Clinical/Enterprise-managed computer vs lab/self-managed computer
  4. Network location (e.g. Public Health, Hospital, and Basic Sciences are all managed as separate networks)
  5. VPN access: typically used from outside Hopkins, but can be done from inside Hopkins; for additional information, see https://cds.johnshopkins.edu/vpn/ (you'll need JHED authentication)

When troubleshooting with a different computer, try to vary one or more of the factors listed.

Asking for help:
Please email us the results of your attempts at troubleshooting, the answers to the factors listed above, and whether your computer(s) have previously been able to reach TempStorage3. Although computer communications can cross many 'jurisdictions' (see [d] above), we will do what we can to help.


Microscope Facility News & Equipment Status

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2017-08-16 (Zeiss LSM 780 FCS): Zeiss 780: argon laser is failing, remains online

8/16/2017 2:12pm (UPDATE)>>780 remains online and available for use
The 780 will remain online and available for use, but with a couple of caveats. Here are some comments/guidelines from Hoku:
- All other laser lines are perfectly functional and require no changes to your current protocols. Anything using the 405, 561, and 633nm lines should be completely unperturbed.
- The 514 line is too degraded to use (unstable power, low output). This means that CFP/YFP FRET experiments are not viable until the new laser head arrives. Furthermore, if you are imaging YFP alone, use the 488 line. We can show you how to modify your protocols.
- The 457 and 488 lines are still working, but at reduced power. You may need to increase the laser power on your specimens to obtain an adequate image in green or CFP.
- If you are doing quantitative imaging using the argon-ion laser (such as measuring raw intensities across specimens), you may wish to wait until the new laser head arrives. However, for most uses (such as determining the localization of a protein, monitoring cell movement or morphology, etc), you will be able to continue to use the system uninterrupted, turning the power up for now and turning it back down again when the new laser arrives.
If you have any questions, please don’t hesitate to ask one of our staff members. Thank you for your patience!

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8/16/2017 2:11pm>>
The argon-ion laser on the LSM 780 (457, 488, and 514nm lines) is failing. A replacement has been ordered, but it will be a few days before it gets in. We apologize for the inconvenience.

2017-07-25 (Zeiss LSM 780 FCS): Zeiss LSM780 BACK ONLINE: part replaced, system recalibrated

7/25/2017 9:28am (UPDATE)>>
As of 9:30am, the 780 is back online. All user reservations from Wed, July 19 to Monday, July 24 have been canceled. Thank you for your patience!

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7/24/2017 4:34pm (UPDATE)>>Zeiss LSM780 now in repair, down until Tuesday 7/25
The Zeiss engineer has replaced the part on the 780, but as of 4:30pm, he is still recalibrating the instrument. Even if he is finished by tonight, Barbara or Hoku will have to inspect the system tomorrow morning before we can bring the 780 back online for our users.

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7/20/2017 11:56am (UPDATE)>>
The 780 will be down until Monday. Unfortunately, that is the earliest date that we can expect to receive parts and service. We really appreciate your patience.

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7/19/2017 2:42pm
The Zeiss LSM 780 is down. Multiple errors are appearing in the software but it may be a hardware issue. We are awaiting a phone call/visit from Zeiss and we will try to keep you updated. We will cancel all reservations from today so please do not be concerned that you will be charged for unused time. Thank you for your patience.

2017-07-05: New server TempStorage4

The Microscope Facility has a new server for its users: TempStorage4 (>3x bigger & >6x faster). Please delete your shortcut to the old server and create a shortcut to the new one at \\162.129.230.229\TempStorage4 (see instructions if you've forgotten how). The login and password to the new server is the same as before. Please copy your data from the old server (accessible as a read-only subdirectory 'TempStorage3' on TempStorage4) to the new server.

6/30/2017 11:41am>>
TempStorage3 Offline/to Retire
TempStorage3 is offline while we bring up the new Server (Tempstorage4), which is >3x bigger and >6x faster. You will need to edit your shortcuts to the new folder (Windows: "\\162.129.230.229\Tempstorage4"; Mac: "162.129.230.229/Tempstorage4"), noting that the 's' is lowercase. The login/password is the same as before for TempStorage3, but will no longer be accessible.

If you don't know how to create such shortcuts, see FastFacts/Network.

Direct access to TempStorage3 (SMB1 or FTP) will no longer be available. Your old data will still be accessible through the new server as a linked folder called 'TempStorage3' and you can copy your old data folder from the old server to the new server. Eventually, TempStorage3 will shut down and retire (linked folder will disappear), but you'll have at least one month to transfer your data.

2017-06-28 (Philips CM120 TEM): Philips TEM, back online, high tension issue resolved

6/28/2017 1:12pm (UPDATE)>>
The Philips TEM is back online. The FEI service engineer just left. The system is back online and operable. New parts have been ordered to resolve this recurring issue (high tension going out). Thank you for your patience! Barbara

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6/28/2017 9:53am>>
Philips TEM OFFLINE, high tension is out
As of this morning, the Philips TEM is offline. According to Barbara, the high tension is out and won’t come on. She has called the engineer who is expected later today. We have no estimate on repair time.

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