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2017-07-14: Problems getting to TempStorage4?

Although most of our users have transitioned to TempStorage4 successfully (see FastFacts/Network for instructions), some are still having issues.

Troubleshooting:

Try to use a different computer to access TempStorage4. Factors that might matter are:

  1. Network type: WiFi (NOT GuestNet!) vs Wired Ethernet (preferred)
  2. Operating system: Windows vs Mac, which version (NOT WinXP!)?
  3. Computer: Clinical/Enterprise-managed computer vs lab/self-managed computer
  4. Network location (e.g. Public Health, Hospital, and Basic Sciences are all managed as separate networks)
  5. VPN access: typically used from outside Hopkins, but can be done from inside Hopkins; for additional information, see https://cds.johnshopkins.edu/vpn/ (you'll need JHED authentication)

When troubleshooting with a different computer, try to vary one or more of the factors listed.

Asking for help:
Please email us the results of your attempts at troubleshooting, the answers to the factors listed above, and whether your computer(s) have previously been able to reach TempStorage3. Although computer communications can cross many 'jurisdictions' (see [d] above), we will do what we can to help.


Microscope Facility News & Equipment Status

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2017-10-13 (Zeiss LSM 510-meta #2): Zeiss 510-meta, computer/network issue, scope inoperable

The 510 computer operation system is corrupted and unrepairable by the Zeiss engineer. We are looking into other options (using a spare computer) and other attempts (BSNO service). Both are in action, but neither will be completed before next Monday.

2017-10-13 (Olympus Station 1 (inverted)): Olympus 1 (inverted): controller box not working, in repair until Mon Oct 16

The Olympus inverted microscope system is unavailable until Mon, Oct 16. It appears that liquid, which was spilled on the controller, leaked down into the electronic circuit board. This may have been causing a malfunction in the operation of the microscope. Scot has cleaned off the circuit board and will reinstall it when it is completely dry. I am sorry for last minute notice and for any inconvenience to you all.

2017-09-19 (Zeiss LSM 510-meta #2): Zeiss LSM 510-Meta (#2) >> Back online, safety bypass switch was the issue

9/20/2017 4:01pm (UPDATE)>> Back online, safety bypass switch was the issue
The 510 system has been serviced and is back online. Thank you for your patience.

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9/19/2017 2:12pm Zeiss LSM 510 Meta #2 Not Available
The Zeiss LSM 510 Meta #2 is not available until further notice due to a broken main shutter component. We will notify all users once we have an ETA on repairs.

2017-09-18 (Zeiss LSM 780 FCS): UPDATE >> Replacement argon laser for 780 has arrived, installation scheduled for Mon Sept 18

9/18/2017 9:41am (UPDATE)>>The replacement argon laser for the 780 will be installed the Zeiss engineer today. Thank you for your patience.

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8/30/2017 9:12am (UPDATE)>>As of today, the argon laser on the 780 has become intermittent and therefore has been turned off. Any samples that need to be excited with 457, 477, 488 and 514 (i.e. CFP, GFP, YFP) will not work on the 780.

The new laser is en route but delayed in customs. We apologize for the inconvenience and will send another update as soon as we have more information.

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8/21/2017 5:00pm>>The replacement part is backordered, however, the Zeiss 780 remains online and available for use. Thank you for your patience!

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8/16/2017 2:12pm (UPDATE)>>780 remains online and available for use
The 780 will remain online and available for use, but with a couple of caveats. Here are some comments/guidelines from Hoku:
- All other laser lines are perfectly functional and require no changes to your current protocols. Anything using the 405, 561, and 633nm lines should be completely unperturbed.
- The 514 line is too degraded to use (unstable power, low output). This means that CFP/YFP FRET experiments are not viable until the new laser head arrives. Furthermore, if you are imaging YFP alone, use the 488 line. We can show you how to modify your protocols.
- The 457 and 488 lines are still working, but at reduced power. You may need to increase the laser power on your specimens to obtain an adequate image in green or CFP.
- If you are doing quantitative imaging using the argon-ion laser (such as measuring raw intensities across specimens), you may wish to wait until the new laser head arrives. However, for most uses (such as determining the localization of a protein, monitoring cell movement or morphology, etc), you will be able to continue to use the system uninterrupted, turning the power up for now and turning it back down again when the new laser arrives.
If you have any questions, please don’t hesitate to ask one of our staff members. Thank you for your patience!

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8/16/2017 2:11pm>>Argon laser is off (CFP/GFP/YFP will not work), remains online, awaiting replacement laser.

The argon-ion laser on the LSM 780 (457, 488, and 514nm lines) is failing. A replacement has been ordered, but it will be a few days before it gets in. We apologize for the inconvenience.

2017-09-15 (Philips CM120 TEM): New Rules (repaired specimen holder)

In response to abuse/breaking the Philips specimen holder:
Effective Mon Sept 18, 2017:

  1. Do NOT use the specimen holder without re-training
  2. EITHER:
    SCHEDULE a short re-training (<15min, FREE),   OR
    WAIT for staff availability to re-train you at the start of your session.
  3. We will SUSPEND/RESTRICT future privileges of anyone operating the instrument without re-training after 9/18/17.

2017-09-05 (Philips CM120 TEM): Philips CM120 TEM Not Available: Grid Holder Broken

The Philips CM120 TEM is not available for use until further notice. The grid holder is broken. Please be careful with the microscope! Carelessness is COSTLY! We now have to wait for this one to be repaired or a replacement ordered. We will email and post a news item as soon as we have new information.

2017-08-29 (3i Spinning Disk Confocal,Leica Cryostat,Leica EM ICE High Pressure Freezer): Physiol G05 Plumbing Repair

9-5-17 11:00AM As of today, all equipment in G05 is functioning and available.

The sewage/drain lines in the ceiling of Physiology G05 have to be replaced. In preparation, we have to shut down ALL equipment in the room: HPF, cryostat, ultramicrotome, chromium evaporator, 3i spinning disk and rotary evaporator. Expected down time for equipment is Tue 8/29 to Tue 9/5.

Thank you for your understanding.


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