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2017-07-14: Problems getting to TempStorage4?

Although most of our users have transitioned to TempStorage4 successfully (see FastFacts/Network for instructions), some are still having issues.

Troubleshooting:

Try to use a different computer to access TempStorage4. Factors that might matter are:

  1. Network type: WiFi (NOT GuestNet!) vs Wired Ethernet (preferred)
  2. Operating system: Windows vs Mac, which version (NOT WinXP!)?
  3. Computer: Clinical/Enterprise-managed computer vs lab/self-managed computer
  4. Network location (e.g. Public Health, Hospital, and Basic Sciences are all managed as separate networks)
  5. VPN access: typically used from outside Hopkins, but can be done from inside Hopkins; for additional information, see https://cds.johnshopkins.edu/vpn/ (you'll need JHED authentication)

When troubleshooting with a different computer, try to vary one or more of the factors listed.

Asking for help:
Please email us the results of your attempts at troubleshooting, the answers to the factors listed above, and whether your computer(s) have previously been able to reach TempStorage3. Although computer communications can cross many 'jurisdictions' (see [d] above), we will do what we can to help.


Microscope Facility News & Equipment Status

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2018-09-13 (Hitachi 7600 TEM): Hitachi TEM Offline: Poor vacuum and computer issues

The Hitachi TEM is offline. The Hitachi engineer will make a visit on Monday, Sept 17.

2018-09-01 (Zeiss LSM 700 Confocal,Zeiss LSM 780 FCS,Zeiss LSM 800 GaAsP): Important rate change for Zeiss confocal microscopes

Effective immediately (9/1/2018), all reserved time on the Zeiss confocal microscopes will be charged at the same rate, even when users leave early for specimen issues. More information at https://microscopy.jhmi.edu/Services/change19.html.

2018-08-31 (Nikon Fluorescence Dissection w SLR): Nikon Dissection Scope, temporarily offline

Nikon Dissection Scope has been temporarily moved from Physiology G09. Please email microscopy@jhmi.edu if you need access to the microscope before it is relocated or returned to Physiology G09.

Thank you for your patience!

2018-08-27 (3i Spinning Disk Confocal): 3i SDC, new light source

The mercury lamp on the 3i spinning disk confocal has been replaced with an HXP 120 metal halide lamp for fluorescence imaging. Instructions for lamp operation are posted at the 3i system. All other microscope functions are the same. Please use the microscope as previously instructed.

2018-08-27: LSM 510-meta offline until further notice

As you may know, the Zeiss LSM510 (Meta 2) has been removed from the reservation calendar and will be offline indefinitely.

Meta 2 has had issues ranging from internet connection to user login. The frequency of these issues is increasing, and most recently, the Zen software stopped communicating with the microscope (preventing data acquisition). Zeiss has ended service contracts for LSM510s so we must pursue repairs elsewhere. We are actively exploring our options and will keep you posted. In the meantime, the remaining data files from the 510’s local temp drive are now stored at \\162.129.230.229\TempStorage4\Meta2LocalTemp.

If you are not already familiar with the other confocal systems at the Microscope Facility, please email us.

We apologize to our loyal Meta2 users, and we thank you all for being good community users!

2018-08-17 (Hitachi 7600 TEM,Leo FESEM,Philips CM120 TEM): TEM/SEM online, plumbing repairs completed

8/17/2018 9:47am (UPDATE) >> The EM scopes are back online. Thank you for your patience.

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8/16/2018 12:05pm (UPDATE) >> As of 12noon, Thursday, August 16, the EM scopes are still offline as plumbing repairs are still being made.
Thank you for your patience.

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8/14/2018 4:12PM Electron Microscopes shut down immediately - for plumbing repair

>> The Microscope Facility EM systems, including the Hitachi TEM, Philips TEM and the Leo FESEM will be shut down immediately for a planned water outage scheduled for Wednesday, August 15. This outage is necessary due to a leaking water main line in the Physiology Building. At the earliest, the microscopes will be back online 12noon on Thursday, August 16.

You can leave your reservation on the calendar if you intend to use it when it is back online. Loza will delete all unused reservations during the plumbing repair.

We apologize for the inconvenience.

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