Fully Automated Inverted Microscope for Ratio Imaging
Model Information
- Olympus IX81 equipped with Prior motorized stage and motorized excitation and emission filter wheels and imaging with Hamamatsu C9100-02 front-illuminated EM-CCD camera
Specifications
- Olympus IX81 motorized inverted microscope with fluorescence and DIC optics
- Prior motorized x,y,z stage and external filter wheels work with multiwavelength dichroics for accurate in color registration and imaging speed for FRET
- Hamamatsu Photonics C9100-02 EMCCD camera with up to 100X on-chip electron amplification gain intended for high sensitivity and reduced photon exposure to cells
- Semrock fluorescence filters with the new hard surface coatings that are extremely high contrast and virtually eliminate bleedthrough. Common fluorophores are listed below, but more fluorescence filter sets are available.
- Olympus UIS2 objectives: 4X/ 0.16, 10X/ 0.3, 40X/ 1.3, 60X/ 1.40 oil, 60X/ 1.20 water, and 100X/ 1.4 oil give the brightest, flattest, high-contrast images of any microscope objective and are achromatic to close tolerances over an extraordinary range—350 to 1000 nm. DIC for 10, 40, 60, and 100X lenses.
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Common Fluorophores (More Available)
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Mfg ID
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GFP
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Semrock 3035A
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CFP
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Semrock 2432A
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YFP
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Semrock 2427A
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dsRed
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Semrock 4040B
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CFP/YFP dual
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Chroma 86002 v1
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eGFP/dsRed dual
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Chroma 86007
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