Lumicks C-Trap G2 System
Combination: Optical Tweezers, Laser-scanning Confocal, Microfluidics
Model Information
- Lumicks C-Trap G2
- Cite in your manuscripts (example text): NIH Grant S10OD025221
(Institute: Office of the Director [OD], Office of Research Infrastructure Programs [ORIP])
Installed: Dec 19, 2019
Specifications:
- High-resolution dual optical tweezers
- Acquisition rate: 50 kHz, <0.1pN resolution, <0.3nm resolution
- Independent force-control and/or force-detection
- Optional tandem positioning control of both traps for ease-of-use
- Optional force-clamp to maintain tension between traps
- Integrated & automated 6-channel microfluidic controls
- Laminar flow chamber allows fast biochemical changes
- Subnanometer xyz-positioning control of specimen chamber
- Simultaneous high-speed video (up to 100fps, bright-field)
- Three-color confocal fluorescence (488, 532, 638nm laser lines)
- Fast 200Hz line-scan between optical traps (kymographs)
- 3 laser lines available:
- 488 (solid-state)
- 532 (solid-state)
- 638 (diode laser)
- High-resolution NA 1.4 condenser (oil-immersion only) for particle tracing
- High-resolution Olympus objective 60X/1.30 (water-immersion only, no DIC, no phase)
- High-stability heating control (CHECK: up to 40°C)
Applications
- Ideal for 2-bead trapping experiments
- Force-dependence of DNA-binding by single-molecules
- Forces of molecular motors
- Microfluidics allow easy assembly of multi-molecular complexes using optical tweezers