Nikon/Yokogawa SoRA Super-Resolution SDC

Nikon Eclipse Ti2-E with Yokogawa CSU-W1 SoRA Super-Resolution Spinning Disk Confocal

NIH Grant S10OD034393

• As per NIH policy, if instrument usage contributes to the conduct of experiments or the analysis of data, the publication must be linked to the S10 grant via MyNCBI as it would be for any research grant (i.e. R01).  Alternatively, it should be acknowledged in the body of the paper.
• Example acknowledgement text
  Research reported in this publication was supported by the Office of the Director of the NIH under award S10OD0343 to Scot Kuo of the Microscope Facility at The Johns Hopkins University School of Medicine.

Model Information

• Nikon Eclipse Ti2-E Inverted Microscope Body with Yokogawa CSU-W1 SoRA Super-Resolution Spinning Disk Confocal

Applications

• Super-resolution and high-speed Spinning Disk Confocal fluorescence imaging
• Deep imaging of tissues and organoids over large areas

Capabilities

• Super-resolution (FWHM, manufacturer specifications): optical 150nm lateral, 320nm axial; after deconvolution: 120nm lateral, 300nm axial
• Customizable automated acquisition and analysis via integrated JOBS module 
• Integrated Perfect Focus 4 System to address focus drift with time-lapse imaging
• Hamamatsu Orca-FusionBT camera (back-thinned sCMOS sensor with 95% quantum efficiency, 5.5 Mpix, 31 fps)
• Capacity for dual-camera FusionBT system
• Water-immersion refill system for deep imaging of tissues and organoids over large areas

Microscope Specifications