Nikon Spinning Disk Confocal & dSTORM Super-Resolution
Modular & customizable optics/microinjection
Model Information
- Yokogawa Spinning Disk Confocal (CSU10)
- Nikon Eclipse TiE fully motorized inverted microscope
- LED illuminator
- Extra illuminator and camera ports; fully modular and customizable (ask us!)
- Optional: Semi-automatic nuclear and cytoplasmic microinjection of adherent cells with Eppendorf FemtoJet with integrated pump and Eppendorf Injectman 4
Specifications
- Yokogawa CSU10 spinning Nipkow disk with microlenses
- Nikon TiE fully motorized platforms
- Integrated Perfect Focus System to address focus drift with time-lapse imaging
- Solid-state laser lines at 405, 488, 568 and 647 nm (>50mW each line into SDC)
- Slidebook and Elements software for intuitive data acquisition
Applications
- Optical sections or image stack with high contrast and definition
- Best for moderate and bright fluorescence
- Low rate photobleaching/ extended cell viability
- Minute details of points and edges
- Simple-to-use, easy software
- Quantitative 3D analysis
- Quantitative colocalization analysis
System Information
| Microscope Model: | Nikon Eclipse Ti-E |
| Camera Model: | Photometrics Evolve 512 EMCCD |
| Imaging Software: | 3i Slidebook & Nikon NIS-Elements |
| Accessories available: | Tokai-Hit environmental chamber (CO2, humidity, 37°C temperature) |
Microscope Specifications
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Objectives
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| Installed | 20x/0.45 S Plan Fluor (dry) |
| 40x/0.60Ph2 S Plan Fluor (dry) | |
| 100x/1.49 Apo TIRF (oil) | |
| Available by request | 40x/1.3 Plan Fluor (oil) 60x/1.4 Plan-Apo (oil) 100x/1.4 Plan-Apo (oil) |
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Filter sets
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| Eyepiece View | CFP |
| YFP | |
| Camera View (both TIRF & Widefield, filter-wheel on emission) |
CFP, YFP, CFP-YFP FRET |
| GFP, RFP, GFP-RFP FRET | |
| Dual-View | CFP/YFP |
| GFP/RFP | |
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Laser Lines
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| CFP | 445nm (40mW Diode laser) |
| not standard cube | 457nm (Argon laser) |
| not standard cube | 477nm (Argon laser) |
| GFP | 488nm (Argon laser) |
| YFP | 514nm (Argon laser) |
| RFP/dSTORM | 561nm (0.5W fiber laser) |
| AF647/dSTORM | 647nm (0.5W fiber laser) |
Examples
Pole cells in Drosophila germarium
Protein in nuclei of Drosophila embryo