Nikon Ti2 with iLas2 Ring-TIRF
Next-Generation Total Internal Reflection Fluorescence (TIRF) Microscopy
Applications
- Widefield or TIRF fluorescence imaging of membrane proteins, receptor proteins, signalling molecules, cortical cytoskeleton proteins
Special Capabilites
- Circular TIRF (Ring-TIRF) for interference-free low-background excitation (iLas2)
- HILO (also known as near TIRF or oblique-angle epifluorescence)
- TIRF illumination exciting molecules within 100 nm of the coverslip surface
- Integrated Perfect Focus System to address focus drift with time-lapse imaging
System Information
| Microscope Model: | Nikon Eclipse Ti2-E |
| Camera Model: | Hamamatsu FusionBT (95%QE) |
| Imaging Software: | Nikon NIS-Elements |
| Accessories available: | Tokai-Hit environmental chamber (CO2, humidity, 37°C temperature) |
Microscope Specifications
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Objectives
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| Installed | 10x/0.3 Plan Fluor (dry) |
| 60x/1.49 Apo TIRF (oil) | |
| Available by request | 40x/1.3 Plan Fluor (oil) 60x/1.4 Plan-Apo (oil) 100x/1.4 Plan-Apo (oil) |
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Laser Lines
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| CFP | 405nm (50mW solid-state laser) |
| GFP | 488nm (60mW solid-state laser) |
| RFP | 561nm (50mW solid-state laser) |
| AF647 | 640nm (40mW solid-state laser) |