Zeiss LSM800 GaAsP
Single-point, laser scanning confocal microscope for
live-cell studies and wide-field camera
Model Information
Zeiss AxioObserver with LSM800 confocal module with upgraded GaAsP detectors
Installed: Jan 19, 2017
Specifications:
Zeiss AxioObserver inverted microscope with LSM800 confocal module
High-sensitivity GaAsP detectors (more sensitive than the bi-alkali detectors of our LSM700; comparable sensitivity to the GaAsP array on our LSM780)
Definite Focus II module to maintain independent specimen focus at multiple positions (original Definite Focus module could only maintain one focus across multiple positions)
High-speed tiling with wide-field CCD camera; integrated control for acquiring confocal images defined by regions within tiled wide-field preview
Dedicated 37°C heater/CO2/humidity for 35mm dishes, chamber slides, or microtiter plates; large enclosure for stable temperature control
NA 0.55 condenser with DIC for 10X, 20X, 40X, and 63X lenses
Objectives:
5X/0.16 PlanApo (dry, no DIC)
10X/0.45 PlanApo (dry), with DIC
20X/0.80 PlanApo (dry), with DIC
40X/1.30 PlanNeofluar oil, with DIC
40X/1.20 C-Apo water, with DIC, upon request
63X/1.4 PlanApo oil, with DIC
100X/1.4 PlanApo oil, with DIC, upon request
4 laser lines available:
405 (diode laser),
488 (solid-state)
561 (solid-state)
639 (diode laser)
Long-life X-Cite 120LEDBoost illuminator for wide-field fluorescence
Latest Zen software to control microscope and confocal
Zen LE software available for desktop computers in your lab
Analysis with Volocity, Imaris or ImageJ on our analysis workstations
Use LOCI BioFormats to import images into ImageJ
Applications
Scan large specimens (brain & other tissue) using CCD camera and select sub-regions for confocal scanning
Single confocal images or image stacks for 3D viewing
Live-cell imaging with temperature and CO2 control
Photoactivation at 405 nm of caged dyes and other molecules
