Zeiss LSM800 GaAsP
Single-point, laser scanning confocal microscope for
live-cell studies and wide-field camera

Model Information

  • Zeiss AxioObserver with LSM800 confocal module with upgraded GaAsP detectors
  • Installed: Jan 19, 2017

Specifications:

  • Zeiss AxioObserver inverted microscope with LSM800 confocal module
  • High-sensitivity GaAsP detectors (more sensitive than the bi-alkali detectors of our LSM700; comparable sensitivity to the GaAsP array on our LSM780)
  • Definite Focus II module to maintain independent specimen focus at multiple positions (original Definite Focus module could only maintain one focus across multiple positions)
  • High-speed tiling with wide-field CCD camera; integrated control for acquiring confocal images defined by regions within tiled wide-field preview
  • Dedicated 37°C heater/CO2/humidity for 35mm dishes, chamber slides, or microtiter plates; large enclosure for stable temperature control
  • NA 0.55 condenser with DIC for 10X, 20X, 40X, and 63X lenses
  • Objectives:
    • 5X/0.16 PlanApo (dry, no DIC)
    • 10X/0.45 PlanApo (dry), with DIC
    • 20X/0.80 PlanApo (dry), with DIC
    • 40X/1.30 PlanNeofluar oil, with DIC
    • 40X/1.20 C-Apo water, with DIC, upon request
    • 63X/1.4 PlanApo oil, with DIC
    • 100X/1.4 PlanApo oil, with DIC, upon request
  • 4 laser lines available:
    • 405 (diode laser),
    • 488 (solid-state)
    • 561 (solid-state)
    • 639 (diode laser)
  • Long-life X-Cite 120LEDBoost illuminator for wide-field fluorescence
  • Latest Zen software to control microscope and confocal
  • Zen LE software available for desktop computers in your lab
    • Analysis with Volocity, Imaris or ImageJ on our analysis workstations
    • Use LOCI BioFormats to import images into ImageJ

Applications

  • Scan large specimens (brain & other tissue) using CCD camera and select sub-regions for confocal scanning
  • Single confocal images or image stacks for 3D viewing
  • Live-cell imaging with temperature and CO2 control
  • Photoactivation at 405 nm of caged dyes and other molecules
  • Colocalization of different fluorescent signals
  • Speed and versatility

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